Composite

Part:BBa_K1688014:Design

Designed by: Mikael Andersson Schönn   Group: iGEM15_Uppsala   (2015-09-15)


Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23101 promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4182
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
    Illegal NotI site found at 8225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5275
    Illegal BamHI site found at 3260
    Illegal BamHI site found at 5962
    Illegal XhoI site found at 1371
    Illegal XhoI site found at 3759
    Illegal XhoI site found at 3911
    Illegal XhoI site found at 9413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1927
    Illegal PstI site found at 5922
    Illegal NgoMIV site found at 1864
    Illegal NgoMIV site found at 3639
    Illegal NgoMIV site found at 5513
    Illegal NgoMIV site found at 6125
    Illegal NgoMIV site found at 9438
    Illegal AgeI site found at 702
    Illegal AgeI site found at 3633
    Illegal AgeI site found at 6341
    Illegal AgeI site found at 8336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2981
    Illegal BsaI site found at 3654
    Illegal BsaI site found at 4245
    Illegal BsaI site found at 5411
    Illegal BsaI.rc site found at 2815
    Illegal BsaI.rc site found at 5565
    Illegal BsaI.rc site found at 7850
    Illegal SapI.rc site found at 3345
    Illegal SapI.rc site found at 3891
    Illegal SapI.rc site found at 7742


Design Notes

The pathway contains two endogenous restriction sites for the enzyme PstI.


Source

Nah7 plasmid from the PpG7 strain of Pseudomonas putida. Standard iGEM registry promoter and RBS.

References